Microvascular free tissue transfer for gene delivery: in vivo evaluation of different routes of plasmid and adenoviral delivery

Transfer of healthy autologous tissue as a microvascular free flap facilitates reconstruction during ablative cancer surgery. In addition to filling surgical defects, free flaps might concentrate viral vectors at the tumour bed and mediate local therapeutic effects. We evaluated the magnitude, topog...

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Veröffentlicht in:	Gene therapy 2009-01, Vol.16 (1), p.78-92
Hauptverfasser:	Agrawal, V K, Copeland, K M, Barbachano, Y, Rahim, A, Seth, R, White, C L, Hingorani, M, Nutting, C M, Kelly, M, Harris, P, Pandha, H, Melcher, A A, Vile, R G, Porter, C, Harrington, K J
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - genetics Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Applied cell therapy and gene therapy Biological and medical sciences Biomedical and Life Sciences Biomedicine Biotechnology Cancer Care and treatment Cell Biology Comparative studies Fundamental and applied biological sciences. Psychology Gene Expression Gene Therapy Genetic aspects Genetic Therapy - methods Genetic transformation Genetic vectors Genetic Vectors - administration & dosage Genetic Vectors - genetics Genetic Vectors - metabolism Health aspects Health. Pharmaceutical industry Human Genetics Industrial applications and implications. Economical aspects Injections Lac Operon Luciferases - analysis Luciferases - genetics Male Medical sciences Methods Models, Animal Nanotechnology original-article Plasmids - pharmacology Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction Surgery Surgical Flaps Tissue Distribution Tissues Transduction, Genetic - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Zusammenfassung:	Transfer of healthy autologous tissue as a microvascular free flap facilitates reconstruction during ablative cancer surgery. In addition to filling surgical defects, free flaps might concentrate viral vectors at the tumour bed and mediate local therapeutic effects. We evaluated the magnitude, topography and duration of luciferase gene expression after plasmid and adenoviral delivery in rat superficial inferior epigastric (SIE) flaps. For plasmid delivery, luciferase expression was significantly increased by all transduction routes (topical, intraflap injection, intravascular) ( P
ISSN:	0969-7128 1476-5462
DOI:	10.1038/gt.2008.140