DNA vaccination of pigs with open reading frame 1–7 of PRRS virus

We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination with ORF...

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Veröffentlicht in:	Vaccine 2004-09, Vol.22 (27), p.3628-3641
Hauptverfasser:	Barfoed, Annette Malene, Blixenkrone-Møller, Merete, Jensen, Merethe Holm, Bøtner, Anette, Kamstrup, Søren
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Antibodies, Viral - biosynthesis Bacteriology Biolistics Biological and medical sciences Cloning, Molecular Deoxyribonucleic acid DNA DNA Primers DNA vaccination Enzyme-Linked Immunosorbent Assay Epitopes - immunology Fundamental and applied biological sciences. Psychology Gene gun Genes Genomics Hogs Immunoenzyme Techniques Mice Microbiology Neutralization Tests Open Reading Frames - immunology Pigs Plasmids - genetics Porcine Reproductive and Respiratory Syndrome - immunology Porcine Reproductive and Respiratory Syndrome - prevention & control Porcine respiratory and reproductive syndrome virus Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - immunology Porcine respiratory and reproductive syndrome virus - isolation & purification PRRS virus Reverse Transcriptase Polymerase Chain Reaction Swine Vaccination - methods Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA - immunology Viral Vaccines - immunology Viremia - blood Viremia - immunology Virology
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Beschreibung
Zusammenfassung:	We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination with ORF7 consistently induced antibodies after three vaccinations, while antibodies were only sporadically detected in the remaining groups. After six vaccinations, all pigs were inoculated with PRRS virus and the post-inoculation antibody response was studied. Pigs vaccinated with ORF1 or ORF4 were primed for antibody response against NSP2 or GP4, respectively. Neutralising antibodies were detected in all pigs, with ORF5 vaccinated pigs showing the highest titres.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2004.03.028