Sol–gel-based poliovirus-1 detector

Hybrid sol–gel films were used to grow Buffalo Green Monkey kidney cell tissues, which were used for poliovirus-1 detection. It is shown that the sol–gel approach allows cutting the standard EPA procedure from 48 to 24 h of detection time; that better visualization of the plaques is obtained; that a...

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Veröffentlicht in:Journal of virological methods 2009-02, Vol.155 (2), p.132-135
Hauptverfasser: Zolkov, Chen, Avnir, David, Armon, Robert
Format: Artikel
Sprache:eng
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Zusammenfassung:Hybrid sol–gel films were used to grow Buffalo Green Monkey kidney cell tissues, which were used for poliovirus-1 detection. It is shown that the sol–gel approach allows cutting the standard EPA procedure from 48 to 24 h of detection time; that better visualization of the plaques is obtained; that a variety of stains, including fluorescence, can be used; and that the shelf life of the resulting plaques system is well over a year.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2008.09.031