Theoretical analysis of dynamic force spectroscopy experiments on ligand–receptor complexes

The forced rupture of single chemical bonds in biomolecular compounds (e.g. ligand–receptor systems) as observed in dynamic force spectroscopy experiments is addressed. Under the assumption that the probability of bond rupture depends only on the instantaneously acting force, a data collapse onto a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biotechnology 2004-08, Vol.112 (1), p.13-23
Hauptverfasser: Raible, M, Evstigneev, M, Reimann, P, Bartels, F.W, Ros, R
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The forced rupture of single chemical bonds in biomolecular compounds (e.g. ligand–receptor systems) as observed in dynamic force spectroscopy experiments is addressed. Under the assumption that the probability of bond rupture depends only on the instantaneously acting force, a data collapse onto a single master curve is predicted. For rupture data obtained experimentally by dynamic AFM force spectroscopy of a ligand–receptor bond between a DNA and a regulatory protein we do not find such a collapse. We conclude that the above mentioned, generally accepted assumption is not satisfied and we discuss possible explanations.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2004.04.017