Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus

1 Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022 Vladivostok, Russia 2 Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd, 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan 3 BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium 4 Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, Pal'chevskogo Street 17, 690032 Vladivostok, Russia Correspondence Olga I. Nedashkovskaya olganedashkovska{at}piboc.dvo.ru Strain KMM 3524 T was isolated from the holothurian Apostichopus japonicus living in the Sea of Japan. The bacterial strain was pigmented, non-motile, Gram-negative, strictly aerobic and oxidase-, catalase- and -galactosidase-positive. From the results of 16S rDNA sequence analysis, strain KMM 3524 T was found to be related closely to Salegentibacter salegens (98·1 %). DNA–DNA homology between strains KMM 3524 T and S. salegens DSM 5424 T was 38 %; this showed clearly that the holothurian isolate KMM 3524 T belongs to a novel species of the genus Salegentibacter for which the name Salegentibacter holothuriorum sp. nov. is proposed, with KMM 3524 T (=NBRC 100249 T =LMG 21968 T ) as the type strain. Published online ahead of print on 9 January 2004 as DOI 10.1099/ijs.0.02987-0. The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of Salegentibacter holothuriorum KMM 3524 T is AB116148 .