Kribbella solani sp. nov. and Kribbella jejuensis sp. nov., isolated from potato tuber and soil in Jeju, Korea

1 KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Suwon, 441-707, Korea 2 School of Biological Sciences, Seoul National University, Seoul, 151-742, Korea 3 Department of Biological Science, Myong Ji University, Yongin 449-728, Korea Correspondence Joo-Won Suh jwsuh{at}mju.ac.kr Two actinomycete strains, DSA1 T and HD9 T , were isolated from a potato tuber and soil from a potato-cultivating field in Jeju, Korea, respectively. A comprehensive 16S rDNA analysis revealed that the isolates belong to the genus Kribbella and share 97·7–98·6 % sequence similarity to Kribbella species. The strains also contained typical chemotaxonomic markers of the genus Kribbella : LL -diaminopimelic acid, alanine, glycine and glutamic acid in the cell wall peptidoglycan; mannose, glucose, galactose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol as characteristic phospholipids; and the major menaquinone MK-9 (H 4 ). DNA–DNA hybridization experiments showed that the isolates represented two distinct genomic species. A number of phenotypic properties can be used to differentiate the two isolates from Kribbella species. On the basis of polyphasic evidence, two novel species are proposed: Kribbella solani sp. nov. for strain DSA1 T (=KACC 20196 T =JCM 12205 T ) and Kribbella jejuensis sp. nov. for strain HD9 T (=KACC 20266 T =JCM 12204 T ). Abbreviations: LL -DAP, LL -diaminopimelic acid Published online ahead of print on 30 April 2004 as DOI 10.1099/ijs.0.02953-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA sequences of strains KACC 20196 T , KACC 20248 T , KACC 20249 T , KACC 20250 T and KACC 20266 T are AY253862 – AY253866 , respectively. An extended neighbour-joining tree and a maximum-parsimony tree for the isolates and related actinomycete taxa are available as supplementary data in IJSEM Online.