Automated Monitoring of Phosphatidylcholine Biosyntheses in Plasmodium falciparum by Electrospray Ionization Mass Spectrometry through Stable Isotope Labeling Experiments
The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was id...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2004-08, Vol.76 (15), p.4515-4521 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4521 |
|---|---|
| container_issue | 15 |
| container_start_page | 4515 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 76 |
| creator | ENJALBAL, Christine ROGGERO, Rodolphe CERDAN, Rachel MARTINEZ, Jean VIAL, Henri AUBAGNAC, Jean-Louis |
| description | The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was identified and quantified through isotopic labeling experiments. A straightforward method based on cone voltage directed in-source fragmentations and relative abundance measurement of endogenous versus deuterated specific fragment ions was developed for simple and rapid automated data acquisition. Such high-throughput analytical protocol allowed us to measure the relative contribution of two different metabolic pathways leading to phosphatidylcholine without performing technically more demanding and time-consuming MS/MS or LC/MS experiments. |
| doi_str_mv | 10.1021/ac049759+ |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_66756112</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17314728</sourcerecordid><originalsourceid>FETCH-LOGICAL-i303t-bd51665049bbe5ecc1f63c3647341e0bae22fd301af61004977537fc94ce75313</originalsourceid><addsrcrecordid>eNqFkc9u1DAQxi0EosvCgRdAFgcuKGDHsZ09tmXbrrQLK22RuFmOM2lckjjYjtTwSDwl7j8OvXCakeanb-b7BqG3lHyiJKeftSHFSvLVx2doQXlOMlGW-XO0IISwLJeEHKFXIVwTQimh4iU6SlDJ-Eos0J_jKbpeR6jxzg02Om-HK-wavG9dGFsdbT13pnWdHQCfWBfmIbYQIGA74H2nQ-9qO_W40Z2xo_aprWa87sBEnwS8nvEm6f5OQm7AOx0CPox3wx6in3FsvZuuWnyIuuoAb4KLbgS81RV0t5esb0bwtochhtfoRdoS4M1DXaLvZ-vL04ts--18c3q8zSwjLGZVzakQPCVSVcDBGNoIZpgoJCsokEpDnjc1I1Q3gpLb4CRnsjGrwkDqKFuiD_e6o3e_JghR9TYY6Do9gJuCEkJyQWn-X5BKRguZkl6i90_Aazf5IZlQOZWlLLkkCXr3AE1VD7Uak23tZ_X4qwRk94ANEW7-zbX_qYRkkqvL_UEVZ1_OD3L3Q31lfwEi36g6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217878570</pqid></control><display><type>article</type><title>Automated Monitoring of Phosphatidylcholine Biosyntheses in Plasmodium falciparum by Electrospray Ionization Mass Spectrometry through Stable Isotope Labeling Experiments</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>ENJALBAL, Christine ; ROGGERO, Rodolphe ; CERDAN, Rachel ; MARTINEZ, Jean ; VIAL, Henri ; AUBAGNAC, Jean-Louis</creator><creatorcontrib>ENJALBAL, Christine ; ROGGERO, Rodolphe ; CERDAN, Rachel ; MARTINEZ, Jean ; VIAL, Henri ; AUBAGNAC, Jean-Louis</creatorcontrib><description>The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was identified and quantified through isotopic labeling experiments. A straightforward method based on cone voltage directed in-source fragmentations and relative abundance measurement of endogenous versus deuterated specific fragment ions was developed for simple and rapid automated data acquisition. Such high-throughput analytical protocol allowed us to measure the relative contribution of two different metabolic pathways leading to phosphatidylcholine without performing technically more demanding and time-consuming MS/MS or LC/MS experiments.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac049759+</identifier><identifier>PMID: 15283596</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Automation - methods ; Chromatography, Liquid ; Deuterium ; Isotopes ; Mass Spectrometry ; Metabolism ; Parasites ; Phosphatidylcholines - biosynthesis ; Plasmodium falciparum - metabolism ; Spectrometry, Mass, Electrospray Ionization</subject><ispartof>Analytical chemistry (Washington), 2004-08, Vol.76 (15), p.4515-4521</ispartof><rights>Copyright American Chemical Society Aug 1, 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15283596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ENJALBAL, Christine</creatorcontrib><creatorcontrib>ROGGERO, Rodolphe</creatorcontrib><creatorcontrib>CERDAN, Rachel</creatorcontrib><creatorcontrib>MARTINEZ, Jean</creatorcontrib><creatorcontrib>VIAL, Henri</creatorcontrib><creatorcontrib>AUBAGNAC, Jean-Louis</creatorcontrib><title>Automated Monitoring of Phosphatidylcholine Biosyntheses in Plasmodium falciparum by Electrospray Ionization Mass Spectrometry through Stable Isotope Labeling Experiments</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was identified and quantified through isotopic labeling experiments. A straightforward method based on cone voltage directed in-source fragmentations and relative abundance measurement of endogenous versus deuterated specific fragment ions was developed for simple and rapid automated data acquisition. Such high-throughput analytical protocol allowed us to measure the relative contribution of two different metabolic pathways leading to phosphatidylcholine without performing technically more demanding and time-consuming MS/MS or LC/MS experiments.</description><subject>Animals</subject><subject>Automation - methods</subject><subject>Chromatography, Liquid</subject><subject>Deuterium</subject><subject>Isotopes</subject><subject>Mass Spectrometry</subject><subject>Metabolism</subject><subject>Parasites</subject><subject>Phosphatidylcholines - biosynthesis</subject><subject>Plasmodium falciparum - metabolism</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EosvCgRdAFgcuKGDHsZ09tmXbrrQLK22RuFmOM2lckjjYjtTwSDwl7j8OvXCakeanb-b7BqG3lHyiJKeftSHFSvLVx2doQXlOMlGW-XO0IISwLJeEHKFXIVwTQimh4iU6SlDJ-Eos0J_jKbpeR6jxzg02Om-HK-wavG9dGFsdbT13pnWdHQCfWBfmIbYQIGA74H2nQ-9qO_W40Z2xo_aprWa87sBEnwS8nvEm6f5OQm7AOx0CPox3wx6in3FsvZuuWnyIuuoAb4KLbgS81RV0t5esb0bwtochhtfoRdoS4M1DXaLvZ-vL04ts--18c3q8zSwjLGZVzakQPCVSVcDBGNoIZpgoJCsokEpDnjc1I1Q3gpLb4CRnsjGrwkDqKFuiD_e6o3e_JghR9TYY6Do9gJuCEkJyQWn-X5BKRguZkl6i90_Aazf5IZlQOZWlLLkkCXr3AE1VD7Uak23tZ_X4qwRk94ANEW7-zbX_qYRkkqvL_UEVZ1_OD3L3Q31lfwEi36g6</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>ENJALBAL, Christine</creator><creator>ROGGERO, Rodolphe</creator><creator>CERDAN, Rachel</creator><creator>MARTINEZ, Jean</creator><creator>VIAL, Henri</creator><creator>AUBAGNAC, Jean-Louis</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Automated Monitoring of Phosphatidylcholine Biosyntheses in Plasmodium falciparum by Electrospray Ionization Mass Spectrometry through Stable Isotope Labeling Experiments</title><author>ENJALBAL, Christine ; ROGGERO, Rodolphe ; CERDAN, Rachel ; MARTINEZ, Jean ; VIAL, Henri ; AUBAGNAC, Jean-Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i303t-bd51665049bbe5ecc1f63c3647341e0bae22fd301af61004977537fc94ce75313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Automation - methods</topic><topic>Chromatography, Liquid</topic><topic>Deuterium</topic><topic>Isotopes</topic><topic>Mass Spectrometry</topic><topic>Metabolism</topic><topic>Parasites</topic><topic>Phosphatidylcholines - biosynthesis</topic><topic>Plasmodium falciparum - metabolism</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ENJALBAL, Christine</creatorcontrib><creatorcontrib>ROGGERO, Rodolphe</creatorcontrib><creatorcontrib>CERDAN, Rachel</creatorcontrib><creatorcontrib>MARTINEZ, Jean</creatorcontrib><creatorcontrib>VIAL, Henri</creatorcontrib><creatorcontrib>AUBAGNAC, Jean-Louis</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ENJALBAL, Christine</au><au>ROGGERO, Rodolphe</au><au>CERDAN, Rachel</au><au>MARTINEZ, Jean</au><au>VIAL, Henri</au><au>AUBAGNAC, Jean-Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated Monitoring of Phosphatidylcholine Biosyntheses in Plasmodium falciparum by Electrospray Ionization Mass Spectrometry through Stable Isotope Labeling Experiments</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>76</volume><issue>15</issue><spage>4515</spage><epage>4521</epage><pages>4515-4521</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was identified and quantified through isotopic labeling experiments. A straightforward method based on cone voltage directed in-source fragmentations and relative abundance measurement of endogenous versus deuterated specific fragment ions was developed for simple and rapid automated data acquisition. Such high-throughput analytical protocol allowed us to measure the relative contribution of two different metabolic pathways leading to phosphatidylcholine without performing technically more demanding and time-consuming MS/MS or LC/MS experiments.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>15283596</pmid><doi>10.1021/ac049759+</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2004-08, Vol.76 (15), p.4515-4521 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66756112 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Automation - methods Chromatography, Liquid Deuterium Isotopes Mass Spectrometry Metabolism Parasites Phosphatidylcholines - biosynthesis Plasmodium falciparum - metabolism Spectrometry, Mass, Electrospray Ionization |
| title | Automated Monitoring of Phosphatidylcholine Biosyntheses in Plasmodium falciparum by Electrospray Ionization Mass Spectrometry through Stable Isotope Labeling Experiments |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T12%3A32%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Automated%20Monitoring%20of%20Phosphatidylcholine%20Biosyntheses%20in%20Plasmodium%20falciparum%20by%20Electrospray%20Ionization%20Mass%20Spectrometry%20through%20Stable%20Isotope%20Labeling%20Experiments&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=ENJALBAL,%20Christine&rft.date=2004-08-01&rft.volume=76&rft.issue=15&rft.spage=4515&rft.epage=4521&rft.pages=4515-4521&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac049759+&rft_dat=%3Cproquest_pubme%3E17314728%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217878570&rft_id=info:pmid/15283596&rfr_iscdi=true |