Automated Monitoring of Phosphatidylcholine Biosyntheses in Plasmodium falciparum by Electrospray Ionization Mass Spectrometry through Stable Isotope Labeling Experiments

The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was id...

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Veröffentlicht in:Analytical chemistry (Washington) 2004-08, Vol.76 (15), p.4515-4521
Hauptverfasser: ENJALBAL, Christine, ROGGERO, Rodolphe, CERDAN, Rachel, MARTINEZ, Jean, VIAL, Henri, AUBAGNAC, Jean-Louis
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Sprache:eng
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Zusammenfassung:The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was identified and quantified through isotopic labeling experiments. A straightforward method based on cone voltage directed in-source fragmentations and relative abundance measurement of endogenous versus deuterated specific fragment ions was developed for simple and rapid automated data acquisition. Such high-throughput analytical protocol allowed us to measure the relative contribution of two different metabolic pathways leading to phosphatidylcholine without performing technically more demanding and time-consuming MS/MS or LC/MS experiments.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac049759+