Simultaneous detection for three kinds of veterinary drugs: Chloramphenicol, clenbuterol and 17-beta-estradiol by high-throughput suspension array technology

Suspension array technology for simultaneous detection of three kinds of veterinary drugs, chloramphenicol (CAP), clenbuterol and 17-beta-estradiol has been developed. Conjugates of chloramphenicol and clenbuterol coupled with bovine serum albumin were synthesized and purified. Probes of suspension...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytica chimica acta 2009-01, Vol.632 (1), p.128-134
Hauptverfasser: Liu, Nan, Su, Pu, Gao, Zhixian, Zhu, Maoxiang, Yang, Zhihua, Pan, Xiujie, Fang, Yanjun, Chao, Fuhuan
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Suspension array technology for simultaneous detection of three kinds of veterinary drugs, chloramphenicol (CAP), clenbuterol and 17-beta-estradiol has been developed. Conjugates of chloramphenicol and clenbuterol coupled with bovine serum albumin were synthesized and purified. Probes of suspension array were constituted by coupling the three conjugates on the fluorescent microspheres/beads and the microstructures of the beads’ surface were observed by scanning electron microscopy which was a direct confirmation for the successful conjugates’ coupling. The optimal addition of conjugates and the amounts of antibodies were optimized and selected, respectively. Standard curves were plotted and the coefficient of determination- R 2 was greater than 0.989 which suggested good logistic correlation. The detection ranges for the three veterinary drugs are 40–6.25 × 10 5 ng L −1, 50–7.81 × 10 5 ng L −1 and 1 × 10 3–7.29 × 10 5 ng L −1, respectively and the lowest detection limits (LDLs) of them are 40, 50 and 1000 ng L −1, respectively. The suspension array is specific and has no significant cross-reactivity with other chemicals. Meanwhile, unknown samples were detected by suspension array and ELISA in comparison with each other. The errors between found and real for the detection of the unknown samples were relatively small to both of the two methods, whereas, the detection ranges of suspension array are broader and sensitive than that of the traditional ELISA. The high-throughput suspension array is proved to be a novel method for multi-analysis of veterinary drugs with simple operation, high sensitivity and low cost.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2008.10.061