First record of Perkinsus olseni, a protozoan parasite infecting the commercial clam Ruditapes decussatus in Spanish Mediterranean waters

We present the first record in Spanish Mediterranean waters of the protozoan parasite Perkinsus olseni infecting the clam Ruditapes decussatus. Perkinsus infection was detected all year around albeit at a low level of infection intensity. Histological analysis, induction of zoospores and in situ hyb...

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Veröffentlicht in:	Journal of invertebrate pathology 2009, Vol.100 (1), p.50-53
Hauptverfasser:	Elandaloussi, Laurence M., Carrasco, Noèlia, Roque, Ana, Andree, Karl, Dolores Furones, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal aquaculture animal diseases animal pathogens Animal productions Animals Biological and medical sciences Bivalvia - cytology Bivalvia - parasitology Carpet-shell clam clams disease incidence FTM Fundamental and applied biological sciences. Psychology Histology histopathology In situ hybridisation Invertebrate aquaculture Invertebrates Life cycle. Host-agent relationship. Pathogenesis Marine Mediterranean Sea microbial genetics molecular sequence data Mollusca new geographic records nucleic acid hybridization nucleotide sequences Parasites - classification Parasites - genetics Parasites - isolation & purification pathogen identification Pathology PCR Perkinsus Perkinsus olseni polymerase chain reaction Protozoa protozoal infections Protozoan parasite RNA, Ribosomal - chemistry Ruditapes decussatus sequence analysis Sequence Analysis, RNA Spain zoospores
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Zusammenfassung:	We present the first record in Spanish Mediterranean waters of the protozoan parasite Perkinsus olseni infecting the clam Ruditapes decussatus. Perkinsus infection was detected all year around albeit at a low level of infection intensity. Histological analysis, induction of zoospores and in situ hybridisation assay confirmed the presence of Perkinsus sp. The identity of the parasite was determined by species-specific PCR assay in DNA samples obtained from infected clams. Sequencing of amplified fragments showed 100% identity to the ITS region of P. olseni. We confirmed for the first time the presence of P. olseni in Spanish Mediterranean waters.
ISSN:	0022-2011 1096-0805
DOI:	10.1016/j.jip.2008.09.004