Assessment of a microplate method for detection of staphylococcal secretory inhibitor of platelet microbicidal protein

Abstract We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in ≤24 h. The levels of SIPMP production agreed with the results obtained by viable counting method. Both methods may be acceptable and c...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2009-01, Vol.63 (1), p.118-120
Hauptverfasser:	Ivanov, Iuri B, Gritsenko, Viktor A
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - pharmacology Bacteriological Techniques - methods beta-Thromboglobulin - antagonists & inhibitors Blood Bactericidal Activity - drug effects Blood Platelets - metabolism Chronic Disease Humans Infectious Disease Internal Medicine Male Platelet microbicidal protein Prostate - microbiology Prostatitis - microbiology Reproducibility of Results Sensitivity and Specificity Spectrophotometry - methods Staphylococcal Infections - microbiology Staphylococci Staphylococcus - metabolism
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Zusammenfassung:	Abstract We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in ≤24 h. The levels of SIPMP production agreed with the results obtained by viable counting method. Both methods may be acceptable and cheap, allowing for their routine use in clinical laboratories.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2008.09.011