Real-time quantitative assay of telomerase activity using the duplex scorpion primer

A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 10(4) cells expressing telomerase act...

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Veröffentlicht in:	Biotechnology letters 2004-06, Vol.26 (11), p.891-895
Hauptverfasser:	Huang, Yanping, Kong, Deming, Yang, Yi, Niu, Ruifang, Shen, Hanxi, Mi, Huaifeng
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging Biological and medical sciences Biomarkers, Tumor - analysis Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biotechnology DNA Primers Enzyme Activation Enzymes Fluorescence Resonance Energy Transfer - methods Fundamental and applied biological sciences. Psychology HL-60 Cells Humans Reverse Transcriptase Polymerase Chain Reaction - methods Telomerase Telomerase - analysis Telomerase - genetics Telomerase - metabolism
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Beschreibung
Zusammenfassung:	A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 10(4) cells expressing telomerase activity could be obtained (R2 = 0.994). The requirement of post-PCR steps is thus obviated.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/B:bile.0000025898.15309.0e