Production of an anti-complement exo-polymer produced by Auricularia auricula-judae in submerged culture
Exo-polymer (EP) was produced at 1.2 g l(-1) in submerged culture of Auricular auricula-judae. Crude EP (AJ-0) has 70% anti-complementary activity (inhibition of total complementary hemolysis 50%; ITCH50). The activating pathway of the complement system occurred through both the classical and altern...
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Veröffentlicht in: | Biotechnology letters 2004-06, Vol.26 (11), p.923-927 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Exo-polymer (EP) was produced at 1.2 g l(-1) in submerged culture of Auricular auricula-judae. Crude EP (AJ-0) has 70% anti-complementary activity (inhibition of total complementary hemolysis 50%; ITCH50). The activating pathway of the complement system occurred through both the classical and alternative pathways, though the major pathway was the classical one. Fractionation of AJ-0 using Sepharose CL-6B gel chromatography gave three major fractions (AJ-Fr-I, II and III) of which the first was the most active. The mycelial growth and EP production of A. auricula-judae were optimal at pH 6, 25 degrees C and pH 5, 25 degrees C, respectively. |
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ISSN: | 0141-5492 1573-6776 |
DOI: | 10.1023/B:bile.0000025904.21519.3a |