High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis

We report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs unsupervised FLIM with autofocus, aut...

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Veröffentlicht in:	Journal of biophotonics 2008-12, Vol.1 (6), p.514-521
Hauptverfasser:	Talbot, Clifford B., McGinty, James, Grant, David M., McGhee, Ewan J., Owen, Dylan M., Zhang, Wei, Bunney, Tom D., Munro, Ian, Isherwood, Beverly, Eagle, Rob, Hargreaves, Andre, Dunsby, Chris, Neil, Mark A. A., French, Paul M. W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Cercopithecus aethiops COS Cells Fluorescence lifetime imaging Fluorescence Resonance Energy Transfer - instrumentation Fluorescent Dyes high content analysis Humans Microscopy, Confocal - instrumentation Microscopy, Fluorescence - instrumentation Protein Interaction Mapping - instrumentation time-resolved imaging unsupervised acquisition
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Beschreibung
Zusammenfassung:	We report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs unsupervised FLIM with autofocus, automatic setting of acquisition parameters and automated localisation of cells in the field of view. We demonstrate its applications to test dye solutions, fixed and live cells and FLIM‐FRET. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:	1864-063X 1864-0648
DOI:	10.1002/jbio.200810054