Expanded Coverage of the Human Heart Mitochondrial Proteome Using Multidimensional Liquid Chromatography Coupled with Tandem Mass Spectrometry

Expanded Coverage of the Human Heart Mitochondrial Proteome Using Multidimensional Liquid Chromatography Coupled with Tandem Mass Spectrometry

Recent evidence suggests that mitochondria are closely linked with the aging process and degenerative disorders such as Alzheimer's disease and Parkinson's disease. Thus, there has been increasing interest in cataloging mitochondrial proteomes to identify potential diagnostic and therapeut...

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Veröffentlicht in:Journal of proteome research 2004-05, Vol.3 (3), p.495-505
Hauptverfasser: Gaucher, Sara P, Taylor, Steven W, Fahy, Eoin, Zhang, Bing, Warnock, Dale E, Ghosh, Soumitra S, Gibson, Bradford W
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, Liquid
Gas Chromatography-Mass Spectrometry
Humans
Mitochondria, Heart - metabolism
Myocardium - metabolism
Proteome
Spectrometry, Mass, Electrospray Ionization
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Zusammenfassung:Recent evidence suggests that mitochondria are closely linked with the aging process and degenerative disorders such as Alzheimer's disease and Parkinson's disease. Thus, there has been increasing interest in cataloging mitochondrial proteomes to identify potential diagnostic and therapeutic targets. We have previously reported results of a one-dimensional electrophoresis/liquid chromatography MS/MS study to characterize the proteome of normal human heart mitochondria (Taylor et al. Nat. Biotechnol. 2003, 21, 281−286). We now report two subsequent studies where multidimensional liquid chromatography MS/MS was investigated as an alternative means for characterizing the same sample. Keywords: mitochondria • LC/MS/MS • MudPIT • MDLC
ISSN:1535-3893
1535-3907
DOI:10.1021/pr034102a