A paucity of structural integrity in cloned porcine blastocysts produced in vitro

The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE...

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Veröffentlicht in:	Theriogenology 2004-09, Vol.62 (5), p.779-789
Hauptverfasser:	Koo, Deog-Bon, Kang, Yong-Kook, Park, Jung Sun, Park, Jin-Ki, Chang, Won-Kyong, Lee, Kyung-Kwang, Han, Yong-Mahn
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blastocyst Blastocyst - cytology Blastocyst - physiology Cell Count Cloning, Organism Differential staining embryo culture embryogenesis embryonic mortality embryonic structures Female Fertilization in Vitro - veterinary In vitro fertilization inner cell mass Male Nuclear transfer Nuclear Transfer Techniques nuclear transplantation Porcine Swine trophectoderm trophoblast
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Beschreibung
Zusammenfassung:	The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE cells was examined and compared with IVF- and in vivo-derived embryos. NT-derived embryos had a lower developmental competence to the blastocyst stage than IVF-derived embryos ( P
ISSN:	0093-691X 1879-3231
DOI:	10.1016/j.theriogenology.2003.12.027