Chapter 7. Nonsense-mediated mRNA decay in Caenorhabditis elegans

Chapter 7. Nonsense-mediated mRNA decay in Caenorhabditis elegans

The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This...

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Veröffentlicht in:Methods in enzymology 2008, Vol.449, p.149-164
Hauptverfasser: Longman, Dasa, Arrisi, Pablo, Johnstone, Iain L, Cáceres, Javier F
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Caenorhabditis elegans - genetics
Codon, Nonsense - genetics
RNA Interference - physiology
RNA Stability - genetics
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Zusammenfassung:The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This chapter describes the development of transgenic reporters as well as the use of genome-wide RNAi and genetic screens to identify novel components of the NMD pathway in the nematode Caenorhabditis elegans.
ISSN:1557-7988
DOI:10.1016/S0076-6879(08)02407-5