Lack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia

Analysis of developmental plasticity of bone marrow-derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with β-galactosidase and enhanced green fluor...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2004-07, Vol.305 (5680), p.90-93
Hauptverfasser:	Harris, Robert G., Herzog, Erica L., Bruscia, Emanuela M., Grove, Joanna E., Van Arnam, John S., Krause, Diane S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals beta-Galactosidase - biosynthesis beta-Galactosidase - genetics Biological and medical sciences Bone marrow Bone Marrow Cells - physiology Bone Marrow Transplantation Cell Differentiation Cell differentiation, maturation, development, hematopoiesis Cell Fusion Cell hybridization Cell physiology Cells Cobra Cardiotoxin Proteins - pharmacology Donors Elapid Venoms - pharmacology Epithelial cells Epithelial Cells - cytology Epithelial Cells - metabolism Female Fluorescence in situ hybridization Fundamental and applied biological sciences. Psychology Genotype & phenotype Green Fluorescent Proteins Hematopoietic stem cell transplantation Hematopoietic stem cells Hepatocytes Hepatocytes - cytology Hepatocytes - metabolism House mouse Keratinocytes - cytology Keratinocytes - metabolism Keratins - analysis Liver Luminescent Proteins - metabolism Lungs Male Mice Mice as laboratory animals Mice, Transgenic Molecular and cellular biology Muscle Cells - cytology Physiological aspects Purkinje cells Radiation, Ionizing Recombinases - metabolism Recombination, Genetic Reverse Transcriptase Polymerase Chain Reaction Somatic cells Stem cells Stem Cells - cytology Stem Cells - physiology Transplantation X Chromosome Y Chromosome
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Beschreibung
Zusammenfassung:	Analysis of developmental plasticity of bone marrow-derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with β-galactosidase and enhanced green fluorescent protein expression in transgenic mice to identify epithelial cells in the lung, liver, and skin that develop from BMDCs without cell fusion.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1098925