Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium

The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that unde...

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Veröffentlicht in:	The Journal of reproduction and development 2004-06, Vol.50 (3), p.305-313
Hauptverfasser:	Li, Q. (Okayama Univ. (Japan)), Niwa, K, Hunter, M.G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Culture Techniques - methods Cell Nucleus - metabolism CULTURE MEDIA Culture Media - chemistry Estradiol - analogs & derivatives Estradiol - pharmacology Estrogen Antagonists - pharmacology Estrogens - metabolism Female Fulvestrant IN VITRO EXPERIMENTATION IN VITRO FERTILIZATION Male MATURATION OESTROGENS Oocytes - drug effects Oocytes - metabolism OVA Progesterone - metabolism Spermatozoa - metabolism SWINE
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Beschreibung
Zusammenfassung:	The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P
ISSN:	0916-8818 1348-4400
DOI:	10.1262/jrd.50.305