Flow cytometric screening of aldolase catalytic antibodies

High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry using a designed fluorogenic retro-aldol retro-Michael substrate is described. High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic...

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Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2004-08, Vol.14 (15), p.4065-4068
Hauptverfasser:	Shim, Hyunbo, Karlström, Amelie, Touami, Sofia M, Fuller, Roberta P, Barbas, Carlos F
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldol Aldolase Animals Antibodies, Catalytic - pharmacology Biological and medical sciences Catalytic antibody Flow Cytometry - methods Fluorescence-based screening Fructose-Bisphosphate Aldolase - drug effects Fructose-Bisphosphate Aldolase - metabolism Fundamental and applied biological sciences. Psychology Fundamental immunology General aspects Hybridomas Kinetics Molecular immunology Recombinant Proteins - drug effects Recombinant Proteins - metabolism Transfection
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Zusammenfassung:	High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry using a designed fluorogenic retro-aldol retro-Michael substrate is described. High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2004.05.045