Determination of bilayer thickness and lipid surface area in unilamellar dimyristoylphosphatidylcholine vesicles from small-angle neutron scattering curves: a comparison of evaluation methods

Small-angle neutron scattering (SANS) experiments were performed on unilamellar 1,2-dimyristoylphosphatidylcholine (DMPC) vesicles prepared in heavy water by extrusion through polycarbonate filters with 500 A pores. The data obtained at 30+/-0.1 degrees C were evaluated using a five-strip function m...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	European biophysics journal 2004-07, Vol.33 (4), p.328-334
Hauptverfasser:	Kucerka, Norbert, Kiselev, Mikhail A, Balgavý, Pavol
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Computer Simulation Dimyristoylphosphatidylcholine - chemistry Experimental data Heavy water Lipid Bilayers - chemistry Liposomes - chemistry Membrane Fluidity Models, Chemical Models, Molecular Molecular Conformation Neutron Diffraction - methods Polar environments Scattering, Radiation Studies Surface area Surface Properties
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Small-angle neutron scattering (SANS) experiments were performed on unilamellar 1,2-dimyristoylphosphatidylcholine (DMPC) vesicles prepared in heavy water by extrusion through polycarbonate filters with 500 A pores. The data obtained at 30+/-0.1 degrees C were evaluated using a five-strip function model of the bilayer coherent neutron scattering length density, three different approximate form factors describing scattering from vesicles, and different methods of evaluation of the experimental data. It is shown that the results obtained from the SANS data in the range of scattering vector values 0.0316 A(-1)< q
ISSN:	0175-7571 1432-1017
DOI:	10.1007/s00249-003-0349-0