Synthesis and characterisation of novel chromogenic substrates for human pancreatic α-amylase
Graphic Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic α-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4- O-Methylated derivatives of these substrates underwent...
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Veröffentlicht in: | Carbohydrate research 2004-07, Vol.339 (10), p.1727-1737 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Graphic
Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic α-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-
O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of α-amylases, and are useful in rapidly screening for novel α-amylase inhibitors and for subsequent kinetic characterisation. |
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ISSN: | 0008-6215 1873-426X |
DOI: | 10.1016/j.carres.2004.05.019 |