Protective lactogenic immunity conferred by an edible peptide vaccine to bovine rotavirus produced in transgenic plants
1 Instituto de Virología, CICV, INTA-Castelar, CC77, Morón 1708, Buenos Aires, Argentina 2 Consejo Nacional e Investigaciones Científicas y Técnicas (CONICET), Argentina 3 Instituto de Genética E. A. Favret, CICA, INTA-Castelar, Buenos Aires, Argentina 4 Departamento de Biotecnología and Centro de...
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Veröffentlicht in: | Journal of general virology 2004-07, Vol.85 (7), p.1825-1832 |
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Zusammenfassung: | 1 Instituto de Virología, CICV, INTA-Castelar, CC77, Morón 1708, Buenos Aires, Argentina
2 Consejo Nacional e Investigaciones Científicas y Técnicas (CONICET), Argentina
3 Instituto de Genética E. A. Favret, CICA, INTA-Castelar, Buenos Aires, Argentina
4 Departamento de Biotecnología and Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, 28140 Madrid, Spain
5 University of Saskatchewan, VIDO, Saskatoon, SK, Canada, S7N 5E3
Correspondence Manuel V. Borca mborca{at}piadc.ars.usda.gov
Vaccines produced in transgenic plants constitute a promising alternative to conventional immunogens, presenting the possibility of stimulating secretory and systemic immunity against enteric pathogens when administered orally. Protection against enteric pathogens affecting newborn animals requires, in most cases, the stimulation of lactogenic immunity. Here, the group presents the development of an experimental immunogen based on expression of an immunorelevant peptide, eBRV4, of the VP4 protein of bovine rotavirus (BRV), which has been described as harbouring at least one neutralizing epitope as well as being responsible for the adsorption of the virus to epithelial cells. The eBRV4 epitope was efficiently expressed in transgenic alfalfa as a translational fusion protein with the highly stable reporter enzyme -glucuronidase ( GUS), which served as a carrier, stabilized the synthesized peptide and facilitated screening for the higher expression levels in plants. Correlation of expression of the eBRV4 epitope in plants with those presenting the highest GUS activities was confirmed by a Western blot assay specific for the BRV peptide. The eBRV4 epitope expressed in plants was effective in inducing an anti-rotavirus antibody response in adult female mice when administered either intraperitoneally or orally and, more importantly, suckling mice born from immunized female mice were protected against oral challenge with virulent rotavirus. These results demonstrate the feasibility of inducing lactogenic immunity against an enteric pathogen using an edible vaccine produced in transgenic plants.
These authors contributed equally to the results presented in this article.
Present address: Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA. |
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ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/vir.0.19659-0 |