Conversion of olive pomace oil to cocoa butter-like fat in a packed-bed enzyme reactor
Refined olive pomace oil (ROPO) was utilized as a source oil for production of cocoa butter-like fat. Immobilized sn-1,3 specific lipase catalyzed acidolysis of ROPO with palmitic (PA) and stearic (SA) acids was performed in a laboratory scale packed-bed reactor. Effect of reactor conditions on prod...
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Veröffentlicht in: | Bioresource technology 2009, Vol.100 (1), p.324-329 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Refined olive pomace oil (ROPO) was utilized as a source oil for production of cocoa butter-like fat. Immobilized
sn-1,3 specific lipase catalyzed acidolysis of ROPO with palmitic (PA) and stearic (SA) acids was performed in a laboratory scale packed-bed reactor. Effect of reactor conditions on product formation was studied at various substrate mole ratios (ROPO:PA:SA; 1:1:1, 1:1:3, 1:3:3, 1:2:6), enzyme loads (10%, 20%, 40%), substrate flow rates (1.5, 4.5, 7.5, 15
ml/min) and solvent amounts (150, 400
ml). The highest yield (10.9% POP, 19.7% POS and 11.2% SOS) was obtained at 40% enzyme load, 1:2:6 substrate mole ratio, 45
°C, 7.5
ml/min substrate flow rate, 150
ml solvent and 3
h reaction time. The melting profile and SFC of the product were comparable to those of CB. Polarized light microscope (PLM) images showed no drastic changes in polymorphic behavior between CB and product. |
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ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2008.05.035 |