Isolation of 8-hydroxyglycitein and 6-hydroxydaidzein from soybean miso

We isolated from soybean miso 8-hydroxyglycitein and 6-hydroxydaidzein as DPPH-radical scavengers, and elucidated their chemical structures by mass spectrometric, and 1 H- and 13 C-NMR spectrosopic analyses. These compounds showed DPPH-radical scavenging activity as high as that of α-tocopherol, 8-h...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-06, Vol.68 (6), p.1372-1374
Hauptverfasser:	Hirota, A. (Shizuoka Univ. (Japan)), Inaba, M, Chen, Y.C, Abe, N, Taki, S, Yano, M, Kawaii, S
Format:	Artikel
Sprache:	eng
Schlagworte:	8-hydroxyglycitein 8-hydroxyisoflavone Biological and medical sciences Biphenyl Compounds Cell Proliferation - drug effects CELLS CHEMICAL STRUCTURE DAIDZEIN DPPH-radical scavenger FLAVONOIDS Free Radical Scavengers - chemistry Free Radical Scavengers - isolation & purification Free Radical Scavengers - pharmacology FREE RADICALS Fundamental and applied biological sciences. Psychology GENISTEIN Glycine max - chemistry HL-60 cell HL-60 Cells Humans Hydrazines HYDROXIDES Isoflavones - chemistry Isoflavones - isolation & purification Isoflavones - pharmacology ISOLATION MASS SPECTROMETRY Molecular Structure Nuclear Magnetic Resonance, Biomolecular Phytoestrogens - chemistry Phytoestrogens - isolation & purification Phytoestrogens - pharmacology Picrates soybean miso SOYFOODS
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Zusammenfassung:	We isolated from soybean miso 8-hydroxyglycitein and 6-hydroxydaidzein as DPPH-radical scavengers, and elucidated their chemical structures by mass spectrometric, and 1 H- and 13 C-NMR spectrosopic analyses. These compounds showed DPPH-radical scavenging activity as high as that of α-tocopherol, 8-hydroxygenistein and 8-hydroxydaidzein. This is the first report of the isolation of 8-hydroxyglycitein from a natural source.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.68.1372