Purification and cDNA cloning of LaIT2, a novel insecticidal toxin from venom of the scorpion Liocheles australasiae

The novel insecticidal toxin, LaIT2, was isolated from venom of the scorpion Liocheles australasiae. The amino acid sequence of LaIT2 was determined by an Edman degradation analysis and subsequent cDNA cloning. LaIT2 is composed of 59 amino acids with three disulfide bridges, and shares sequence sim...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-12, Vol.73 (12), p.2769-2772
Hauptverfasser:	Matsushita, N., Kyoto Univ. (Japan), Miyashita, M, Ichiki, Y, Ogura, T, Sakuradani, E, Nakagawa, Y, Shimizu, S, Miyagawa, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence AMINO ACID SEQUENCES Animals Base Sequence Biological and medical sciences cDNA cloning CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology http://www.fao.org/aos/agrovoc#c_27503 http://www.fao.org/aos/agrovoc#c_37202 http://www.fao.org/aos/agrovoc#c_3887 http://www.fao.org/aos/agrovoc#c_5691 http://www.fao.org/aos/agrovoc#c_6877 http://www.fao.org/aos/agrovoc#c_7828 http://www.fao.org/aos/agrovoc#c_8187 insect toxicity INSECTICIDAS INSECTICIDE INSECTICIDES Insecticides - chemistry Insecticides - isolation & purification MOLECULAR CLONING Molecular Sequence Data PEPTIDE PEPTIDES PEPTIDOS scorpion toxin Scorpion Venoms - chemistry Scorpion Venoms - genetics Scorpion Venoms - isolation & purification SCORPIONES Scorpions SECUENCIAS DE AMINOACIDOS SEQUENCE D'ACIDES AMINES TOXINAS TOXINE TOXINS VENENOS VENIN VENOMS
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Zusammenfassung:	The novel insecticidal toxin, LaIT2, was isolated from venom of the scorpion Liocheles australasiae. The amino acid sequence of LaIT2 was determined by an Edman degradation analysis and subsequent cDNA cloning. LaIT2 is composed of 59 amino acids with three disulfide bridges, and shares sequence similarity to the scorpion β-KTx peptides.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.90509