Capillary isoelectric focusing coupled offline to matrix assisted laser desorption/ionization mass spectrometry

This work presents several critical details for making cIEF-MALDI-MS a robust technique which will allow for more routine application and aid in automation. This includes emphasis on the hardware necessary for syringe pump mobilization and proper protocol for preventing disruption from gas bubbles....

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Veröffentlicht in:	Journal of Chromatography A 2010, Vol.1217 (1), p.179-182
Hauptverfasser:	Weiss, Noah G., Zwick, Nicole L., Hayes, Mark A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Capillary IEF Cattle Fundamental and applied biological sciences. Psychology General aspects, investigation methods Horses Isoelectric Focusing - methods Lactoglobulins - chemistry MALDI-MS Myoglobin - chemistry Proteins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Syringe pump
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Beschreibung
Zusammenfassung:	This work presents several critical details for making cIEF-MALDI-MS a robust technique which will allow for more routine application and aid in automation. This includes emphasis on the hardware necessary for syringe pump mobilization and proper protocol for preventing disruption from gas bubbles. Following these guidelines, excellent elution time reproducibility is demonstrated for six p I markers (RSD
ISSN:	0021-9673 1873-3778
DOI:	10.1016/j.chroma.2009.11.047