Biodegradation of the analgesic naproxen by Trametes versicolor and identification of intermediates using HPLC-DAD-MS and NMR
The white-rot fungus Trametes vesicolor degraded naproxen (10 mg L −1) in a liquid medium to non-detectable levels after 6 h. When naproxen was added in the range of concentrations typically found in the environment (55 μg L −1), it was almost completely degraded (95%) after 5 h. In vitro degradatio...
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Veröffentlicht in: | Bioresource technology 2010-04, Vol.101 (7), p.2159-2166 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The white-rot fungus
Trametes vesicolor degraded naproxen (10
mg
L
−1) in a liquid medium to non-detectable levels after 6
h. When naproxen was added in the range of concentrations typically found in the environment (55
μg
L
−1), it was almost completely degraded (95%) after 5
h.
In vitro degradation experiments with purified laccase and purified laccase plus mediator 1-hydroxybenzotriazol showed slight and almost complete naproxen degradation, respectively. A noticeable inhibition on naproxen degradation was also observed when the cytochrome P450 inhibitor 1-aminobenzotriazole was added to the fungal cultures. These data suggest that both enzymatic systems could play a role in naproxen degradation. 2-(6-hydroxynaphthalen-2-yl)propanoic acid and 1-(6-methoxynaphthalen-2-yl)ethanone were structurally elucidated by HPLC-DAD-MS and NMR as degradation intermediates of naproxen. After 6
h of incubation, both parent compound and intermediates disappeared from the medium. The non-toxicity of the treated medium was confirmed by Microtox test. |
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ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2009.11.019 |