Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning

The presence of galactose α-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclea...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2002-02, Vol.295 (5557), p.1089-1092
Hauptverfasser:	Lai, Liangxue, Kolber-Simonds, Donna, Park, Kwang-Wook, Cheong, Hee-Tae, Greenstein, Julia L., Im, Gi-Sun, Samuel, Melissa, Bonk, Aaron, Rieke, August, Day, Billy N., Murphy, Clifton N., Carter, David B., Hawley, Robert J., Prather, Randall S.
Format:	Artikel
Sprache:	eng
Schlagworte:	a-1,3-Galactosyltransferase Alleles Animals Animals, Genetically Modified Biological and medical sciences Cell culture techniques Cell Line Cell lines Cells Cloning Cloning, Organism Coding Communicable Diseases Donors Embryo Transfer Embryos Exons Female Fetus Fibroblasts Galactosyltransferases - genetics Gene Targeting Genetic aspects Genetic Vectors Genomics Hogs Laboratory animals Male Medical sciences Mutagenesis, Insertional Narcotics nuclear transfer Nuclear Transfer Techniques Oocytes Physiological aspects Pregnancy Recombination, Genetic Somatic cells Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surrogacy Swine Swine, Miniature - embryology Swine, Miniature - genetics Technology. Biomaterials. Equipments Transfection Transferases Transplantation of organs, tissues, etc Xenotransplantation
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Zusammenfassung:	The presence of galactose α-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1068228