Characterization of reactive oxygen species generated by protoporphyrin IX under X-ray irradiation

As a possible radiosensitizer candidate having biological compatibility, oncotropic property, and X-ray activation capability, contribution of protoporphyrin IX (PpIX) to enhanced generation of reactive oxygen species (ROS) under X-ray and UV irradiations were examined. To identify the kinds of ROS,...

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Veröffentlicht in:Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2009-11, Vol.78 (11), p.889-898
Hauptverfasser: Takahashi, Junko, Misawa, Masaki
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Sprache:eng
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Zusammenfassung:As a possible radiosensitizer candidate having biological compatibility, oncotropic property, and X-ray activation capability, contribution of protoporphyrin IX (PpIX) to enhanced generation of reactive oxygen species (ROS) under X-ray and UV irradiations were examined. To identify the kinds of ROS, 2-[6-(4-amino)phenoxy-3 H-xanthen-3-on-9-yl] benzoic acid (APF) and dihydroethidium (DHE) were used together with ethanol as a hydroxyl radical (OH ) quencher. All of the three species of our interest (OH , superoxide radical (O 2 − ), and singlet oxygen ( 1O 2)) were enhanced by PpIX under X-ray and UV irradiations in addition to those by radiolysis and photolysis. Its enhancement factors exceeded 1.7 depending on the concentrations of PpIX from 1.5 to 15.0 μg/ml.
ISSN:0969-806X
1879-0895
DOI:10.1016/j.radphyschem.2009.06.036