Nanocomposite based on modified TiO@d2-BSA for functional applications

A novel nanocomposite based on TiO@d2-protein for functional applications was prepared and characterized. The composite was made by covalent immobilisation of bovine serum albumin (BSA) on to the TiO@d2 particles. BSA was attached to the TiO@d2 powder through a three-step process. This involves the functionalization of the TiO@d2 particles with silane and the subsequent coupling by glutaraldehyde to free NH@d2 groups of the protein. Formation of Schiff's base was confirmed by IR spectroscopy and the optimum loading of the BSA was found to be 73.61%. Surface morphology of the composite was studied by SEM and TEM. Thermal analysis of TiO@d2-BSA composite was carried out by TGA and DSC. Structural variation of the BSA after immobilisation was studied by CD spectra.