In situ laboratory analysis of sucrose in sugarcane bagasse using attenuated total reflectance spectroscopy and chemometrics

The determination of low-level sucrose residue in sugarcane bagasse was investigated by attenuated total reflectance (ATR) spectroscopy to increase the level of sugar production in on-line sugar processing. The efficacy of ATR spectroscopy and chemometrics is described to determine the sucrose level of 0.5 percentage (w/w) or less. The ATR spectra from a range of sugar concentrations obtained by Fourier transform infrared spectrometer in its finger print region (900-1200 cm⁻¹) were incorporated to compute the calibration model using chemometrics approaches such as partial least square and principal component regression. Through the use of this procedure, a calibration model based on absorbance spectra was computed for sucrose residue with standard errors of calibration of 0.662% and standard of prediction of 0.34% over 0.5-5% range. The correlation coefficient (R²) value of 0.998-0.988 suggested the accuracy of the calibration model. An independent set of twenty samples of sucrose solution was used to reconfirm the modal. Ten sugarcane bagasse samples were prepared in vitro and sucrose concentration were predicted using develop calibration model. The mathematically extracted sucrose concentrations were verified for accuracy by high performance liquid chromatography (HPLC). The results presented demonstrate that described technique is effective to determine sucrose level