The Effect of Collagen Matrix on Calcium Phosphate Mineralization

Mineralization of the proteic matrix composed mostly of collagen type I is controlled by specific interactions of ions Ca2+ and PO43-, present in the biological fluid, with the matrix, and by the diffusion of these ions. The specific interactions and the diffusion of ions combined; result in the nucleation and formation of calcium phosphate particles. Moreover, they control the morphology, size, crystallinity and composition of the particles In this work, precipitation of calcium phosphate particles in a collagen matrix type I was carried out through impregnation of the matrix with a Ca2+ and PO43- solution, with Ca/P ratio 1.67 and pH 2.5. Precipitation of particles associated with matrix structuring was carried out by adsorption of gaseous ammonia.