Activity of Water and Osmotic Coefficients of Histidine Derivatives in Aqueous Solutions at 310.15 K

From the data of vapor pressure osmometry the activity of water, osmotic coefficients, and the values of activity coefficients of two derivatives of histidine: N-Boc-L-histidine (Boc-His-OH, m =0.005–0.14 mol⋅kg −1 ) and N-Boc-L-histidine-methyl ether (Boc-His-OMe, m =0.005–0.05 mol⋅kg −1 ) are obtained in aqueous solutions at 310.15 K. From the comparison of water activity and osmotic coefficient values it follows that Boc-His-OMe shows a more pronounced deviation from ideality than Boc-His-OH. Both components exhibit a stronger non-ideality than histidine and a weaker one than His⋅HCl. By means of potentiometric titration the acid-base properties of Boc-His-OMe are investigated and the ionization constant at 298.15 K is determined. The p K value related to the acid-base equilibrium of the nitrogen atom in the imido group of the imidazole ring is higher (6.47) than the corresponding value of histidine (6.00).