Association of resistin rs1862513, rs10401670 and rs3745367 polymorphisms with resistin level and insulin resistance in an Iranian type 2 diabetic population

Objectives The study investigates the association of single nucleotide polymorphisms (SNP) in resistin gene (RETN) with resistin level, insulin resistance, and the risk of type 2 diabetes in an early diagnosed type 2 diabetic population of Iran. Methods The total of 80 healthy subjects and 80 individuals diagnosed with type 2 diabetes. To ascertain the genotypes of rs1862513 and rs3745367, we performed the polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) technique. The genotyping of resistin rs10401670 was conducted a Tetra-arms amplification system. Resistin and insulin levels were also measured by the ELISA method. Statistical analyses were performed by SPSS software. Results Resistin level was increased significantly in patients with type 2 diabetes; a significant difference was seen in biochemical parameters between diabetic and non-diabetic group except high-density lipoprotein cholesterol (HDL-C), low density lipoprotein (LDL-C), and insulin. We did not find statistically a significant association between type 2 diabetes mellitus [T2DM] and resistin gene at SNP. However, rs3745367 exhibit a significant association with resistin level among individuals with diabetes. The serum resistin level exhibited a positive association level with body mass index, FPG and insulin resistance. Additionally, anegative correlation between resistin levels and age, as well as HDL-C was also revealed (P-value