Dietary Amphora coffeaeformis enhanced growth performance, body composition, biochemical parameters, gene expression on red tilapia (Oreochromis niloticus × O. mossambicus), and resistance to Saprolegnia infection

This study examined how inclusion of the diatom Amphora coffeaeformis in the diet affected Red Tilapia ( Oreochromis niloticus × O. mossambicus ) growth performance, body composition, metabolic parameters, gene expression, and resistance to Saprolegnia infection. Fish weighing an average of 9.0 ± 0.3 g were randomly stocked at a rate of 20 fish per pond (in triplicates), in four equal groups, and fed varied concentrations of A. coffeaeformis at 0% (Control), 1.5%, 3.0%, and 4.5% of diet for 56 days. After the eight-week trial, the fish were infected with Saprolegnia and the cumulative mortality was determined. Amphora coffeaeformis fed fish showed significant ( P