A highly branched glucomannan from the fruiting body of Schizophyllum commune: Structural characteristics and antitumor properties analysis

In this study, a highly branched glucomannan (SCP-1) from Schizophyllum commune fruiting body with good solubility was isolated, and its structural characteristics and antitumor properties were analyzed. The monosaccharides of SCP-1 were fucose, glucosamine hydrochloride, galactose, glucose and mannose with a relative molar ratio of 14:6:210:593:177, and the molecular weight (Mw) of SCP-1 was 15.1 kDa. SCP-1 showed a rough and dense surface, and it was aggregated to particles in distilled water, though it might have triple-helix conformation. The main backbone chain of SCP-1 was →[3)-β-D-Glcp-(1]3→3)-β-D-Glcp-(1→2)-α-D-Manp-(1→2)-α-D-Manp-(1→3)-α-D-Glcp-(1→ and three sides chains including α-D-Glcp-(1→[6)-β-D-Glcp-(1]2→, α-D-Glcp-(1→3)-α-D-Manp-(1→ and α-D-Glcp-(1→[6)-α-D-Galp-(1]3→ were linked with 1,6-glycosidic bond, which was significantly different with the schizophyllan isolated from the mycelia of S. commune. SCP-1 could significantly inhibit the growth of A549 cells, the inhibition rate reached 41.62 % and the percentage of cells in S phase increased from 27.17 % to 56.40 % (400 μg/mL, 48 h). Moreover, SCP-1 could induce cell apoptosis and the total apoptosis rate reached 28.13 %. SCP-1 exerted apoptosis inducing effect probably by reducing the expression ratio of Bcl-2/Bax and the p-PI3K, p-Akt and p-mTOR expression level. The results showed that SCP-1 might have the potential to act as an antitumor agent for lung cancer therapy. •A highly branched glucomannan from the artificially cultivated S. commune were purified.•The structure of SCP-1 contained a main backbone and three sides chains.•SCP-1 structure was significantly different with schizophyllan that previously reported.•SCP-1 significantly inhibited the growth of A549 cells and arrested the cell cycle in S phase.•The cell apoptosis induced by SCP-1 might via inhibition of PI3K/Akt/mTOR signaling pathway.