Copper functionalized, pro-angiogenic, and skin regenerative scaffolds based on novel chitosan/APDEMS modified sepiolite-based formulation

Biomaterials-based scaffolds are extensively explored for their proangiogenic and tissue regenerative abilities. The present study aimed to develop wound healing scaffolds based on chitosan/aminopropyldiethoxymethylsilane (APDEMS) modified sepiolite, loaded with copper (0–0.25 g), characterized by FTIR, SEM, mechanical, TGA and analyzed biomedically. The FTIR and SEM confirmed the silane-induced cross-linking and incorporation of copper leading to better dispersion of individual components in the scaffolds. Based on other physicochemical observations, the best scaffold was CS/MS/Cu0.1 (99.5 % increased Young's modulus compared to chitosan, maximum swelling = 900 %, equilibrium time = 70 min); So, CS/MS/Cu0.1 and 0.25 were chosen for further analysis. The CAM assay showed significantly increased angiogenesis in CS/MS/Cu0.1 and 0.25 groups, lacking any developmental anomalies in chick embryos, at lower copper concentrations. The scaffolds' wound healing potential and in-vivo toxicity were assessed by wound excision and histopathology of various organs in mice, respectively. The rate of wound contraction in the CS/MS/Cu0.1 group was significantly (P