Lycium barbarum oligosaccharide-derived carbon quantum dots inhibit glial scar formation while promoting neuronal differentiation of neural stem cells

Overexpression of glial fibrillary acidic protein (GFAP) in activated astrocytes following spinal cord injury is closely associated with glial scar formation, which harms axonal regrowth. In this study, we prepared ultrasmall cationic carbon quantum dots (CQDs) via one-step hydrothermal carbonization. Lycium barbarum oligosaccharides were used as the carbon source for the first time, and polyetherimide (PEI) and ethylenediamine (ED) were used as cationic reagents. Interestingly, the resultant CQDs show the bioactivity of specifically inhibiting GFAP protein expression, while promoting neuronal marker expression in neural stem cells (NSCs). Furthermore, CQDs together with NSCs can remarkably improve the motor activity of animals after implantation into the transection lesion of the rat spinal cord. Histological analysis confirmed that CQDs can enhance neuronal differentiation of NSCs while inhibiting glial scar formation in vivo. Altogether, this study represents the first report of producing CQDs from oligosaccharides and investigating their impact on NSCs differentiation, thus providing a paradigm for exploring the bioactivity of quantum dots.