Influence of rainfall on size-resolved bioaerosols around a livestock farm

Bioaerosols, capable of transporting microorganisms, can impact human health and agriculture by spreading to nearby communities. Their transmissions are influenced by various factors, including weather conditions and human activities. However, the scarcity of detailed, taxon-specific data on bioaerosols' sizes limits our ability to assess risks associated with bioaerosols' generation and spread. This study examined the composition and size of bioaerosols at a livestock farm and a non-agricultural site, focusing on how bioaerosols evolve at different locations and meteorological conditions. The location had an impact on bioaerosol samples. We conducted 16S rRNA gene amplicon sequencing to identify bacteria genera in bioaerosols. We observed consistently higher concentrations of bioaerosols across all sizes at the livestock farm, and samples from the livestock farm exhibited greater bacterial diversity, where we identified Staphylococcus and Corynebacterium as the most abundant species. The effects of rainfall on bioaerosol diversity are complex, suggesting a dynamic interplay between bioaerosol removal and generation. After rainfall, the bioaerosol fraction of particles larger than 2.5 μm increased by nearly 400% compared to post-rain levels. Conversely, for bioaerosols below 1 μm size, the fraction decreased by 50%. Furthermore, the sequencing results showed that precipitation differentially responded to the abundance of various genera in the bioaerosols. Moreover, even for the same genus, the response to precipitation varied depending on the size of the bioaerosols. Our research reveals how size, location, and environmental conditions influence bioaerosol dynamics, enhancing our understanding of bioaerosol formation and transmission. [Display omitted] •598 individual genes are identified in bioaerosols.•Rainfall increases the bacteria fraction for aerosols larger than 2.5 μm.•Rainfall decreases the bacteria fraction for aerosols smaller than 1 μm.•Using the Kruskal-Wallis test, 8 livestock farm marker genes were revealed.•Each genus in size-resolved bioaerosols reacts differently to environmental change.