Toxicological risk assessment of triadimenol for human exposure, broiler health, and food safety

Triadimenol, a widely used triazole fungicide, leaves residues that pose risks to broiler health, food safety, and human health. Current studies focus on lab animals, leaving limited data regarding its impact on non-target organisms in agricultural ecosystems. Moreover, the doses in current studies often exceed typical agricultural pollution levels of triadimenol. Therefore, this study evaluates the toxic effects of triadimenol by exposing broilers to different concentrations (0.05–20 mg/kg) in their feed for 42 days, assessing growth performance, organ index, hematological parameters, histopathology, jejunum morphology, and tissue residues. The results show that triadimenol contamination at 0.05–20 mg/kg in feed does not significantly affect broiler growth performance. However, the significant changes in hematological parameters suggest the potential hematological toxicity of triadimenol in broilers. Triadimenol at 1 mg/kg or higher induces hepatotoxic and nephrotoxic effects, and significantly alters kidney organ index and histopathology in broilers. Additionally, when the triadimenol contamination level in feed exceeds 1 mg/kg, the residues in edible tissues of broilers exceed the limits set by the EU and China. Overall, our study indicates that even low-dose exposure to triadimenol poses potential risks, highlighting the need for strict regulation of its use in agriculture to protect food safety and human health. •Triadimenol at 1 mg/kg or above causes hepatotoxicity and nephrotoxicity in broilers.•Hematological toxicity is observed even at low doses of triadimenol contamination.•Triadimenol residues in broiler exceed safe limits as contamination exceeds 1 mg/kg.•Strict regulation of triadimenol is required to protect food safety and human health.