Whole-cell biosensor engineering based on the transcription factor XylS/Pm for sensitive detection of PCB intermediate chlorobenzoic acid

Whole-cell biosensor engineering based on the transcription factor XylS/Pm for sensitive detection of PCB intermediate chlorobenzoic acid

Chlorobenzoic acids (Cba) are toxic chloride pollutants commonly released by chemical industries or formed during the degradation pathway of organic chlorides, such as polychlorinated biphenyls (PCBs). Here, we successfully developed a highly sensitive Escherichia coli whole-cell Cba biosensor, name...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical engineering journal 2024-02, Vol.202, p.109153, Article 109153
Hauptverfasser: Xian, Yufan, Zhang, Faying, Wang, Meixing, Zhao, Xiang, Sun, Xuting, Lu, Zhenghui, Zhang, Guimin
Format: Artikel
Sprache:eng
Schlagworte:
active sites
biosensors
chlorides
detection limit
Escherichia coli
gene regulatory networks
molecular dynamics
polychlorinated biphenyls
Pseudomonas putida
signal-to-noise ratio
transcription factors
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Chlorobenzoic acids (Cba) are toxic chloride pollutants commonly released by chemical industries or formed during the degradation pathway of organic chlorides, such as polychlorinated biphenyls (PCBs). Here, we successfully developed a highly sensitive Escherichia coli whole-cell Cba biosensor, named XyF10 (MG1655(DE3)-Ps-XylS (Step13)-Pm1-EGFP), by comprehensive engineering the elements of Cba sensing genetic circuit from Pseudomonas putida. Compared to the native sensing system, XyF10 exhibited a significantly reduced detection limit (
ISSN:1369-703X
DOI:10.1016/j.bej.2023.109153