Prevalence, seasonal variation, and proteomic relationship of β-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Acinetobacter spp. in poultry meat at the abattoir level in Greece

Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing Enterobacterales and Acinetobacter spp. are important nosocomial pathogens that are frequently isolated from patients and food matrices. Nevertheless, comprehensive data on the prevalence, spatiotemporal variations, and characterization of β-lactam-resistant bacteria in poultry meat products are limited. This study provides the first comprehensive assessment in Greece of the prevalence, characteristics, and proteomic relationships of β-lactam-resistant strains in poultry meat at the abattoir level. Strains were selectively isolated using β-lactams and identified via MALDI-TOF MS. Antimicrobial susceptibility and the presence of common β-lactamase genes were assessed, and protein profiles were analyzed to determine strain relationships, whereas E. coli isolates were further classified into phylogenetic groups. The overall prevalence was 40.8% for E. coli, 3.3% for K. pneumoniae, and 46.7% for Acinetobacter spp., with notable seasonal and regional fluctuations especially in Acinetobacter spp. Most strains (97.9% of E. coli, 100.0% of K. pneumoniae and 88.1% of Acinetobacter spp.) were classified as multidrug or extensively drug-resistant. All E. coli and K. pneumoniae strains were phenotypically confirmed as ESBL/AmpC producers, whereas one K. pneumoniae strain showed additional resistance to ertapenem. The majority of E. coli strains (91.49%) and all K. pneumoniae strains carried β-lactamase genes, predominantly blaCTX-M group 1 in E. coli and blaSHV in K. pneumoniae. Conversely, only 10.2% of Acinetobacter strains harbored β-lactamase genes. Most E. coli isolates belonged to phylogroups A (46.9%) and B1 (34.7%). Protein profile analysis indicated relatedness among isolates across different regions and seasons. These findings underscore poultry meat's role as a reservoir of resistant strains of E. coli, K. pneumoniae, and Acinetobacter spp. and highlight the need for enhanced surveillance and mitigation strategies to reduce public health risks. •High prevalence of resistant E. coli and Acinetobacter spp. in poultry meat.•One K. pneumoniae isolate demonstrated resistance to ertapenem.•Most strains were multidrug-resistant and harbored numerous β-lactamase genes.•CTX-M of group 1 and SHV predominated in E. coli and K. pneumoniae, respectively.•Close relationship was observed among isolates from different regions and seasons.