Label-free quantitative proteomics to exploit the impact of sourdough fermentation on reducing wheat allergenic fractions

•The microbiota induced by sourdough type II affects the protein expression.•The presence of unique proteins varied according to the LAB applied as a starter culture.•The pH has an important impact on the action of wheat proteases, changing protein profile.•Lactic and acetic acids from fermentation may have a crucial role in reducing allergenicity. The microbial consortia of lactic acid bacteria and yeast of sourdough can partially degrade gluten subunits associated with wheat-related diseases. This study evaluated how sourdough fermentation interferes with wheat protein profiles and if it can be related to the reduction expression of allergenic proteins. Samples from five bread doughs (Saccharomyces cerevisiae -C1; chemical acidification -C2, and three sourdoughs formulations -S1, S2, and S3) were sequentially extracted, digested, and submitted to shotgun label-free proteomic analysis. Eight-five proteins were identified as allergenic, mainly belonging to gliadin fraction, including seven containing the 33-mer peptide sequence. The highest immunogenic potential was found in dough C1 and S3, while the least reactive group consisted of S1 and C2. The two folds down expression of an α-gliadin containing the 33-mer sequence corroborates this. This finding may indicate the role of organic acids produced by the microbiota sourdough type II during fermentation in changing the protein profile.