Preparation and application of multiple particle binding-liposomes for electrochemiluminescent signal amplification in bioassays

In this study, multiple particle binding-liposomes (MPB-Lips), encapsulating the luminophore tris(2′,2-bipyridyl)ruthenium (II) complex ([Ru(bpy) 3 ] 2+ ), were developed as an electrochemiluminescence (ECL) signal amplifier and were applied to detect the model analyte streptavidin (SA) using the in...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2024-11, Vol.416 (28), p.6451-6461
Hauptverfasser:	Tanaka, Aki, Konishi, Atsuko, Takegami, Shigehiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplifiers Analytical Chemistry Binding Bioassays Biochemistry Biological Assay - methods Biomarkers Biosensing Techniques - methods Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Crystals detection limit Electrochemical Techniques - instrumentation Electrochemical Techniques - methods Electrochemiluminescence Electrodes fetal bovine serum Food Science Frequency shift Gold Gold - chemistry Gold coatings Immunoassay Influenza Laboratory Medicine Limit of Detection Lipids Liposomes Liposomes - chemistry Luminescent Measurements - methods Monitoring/Environmental Analysis Nanoparticles Peptides Phospholipids Quartz Quartz crystal microbalance Quartz Crystal Microbalance Techniques - methods Quartz crystals Recovery Research Paper Ruthenium standard deviation Streptavidin Streptavidin - chemistry Surfactants
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Zusammenfassung:	In this study, multiple particle binding-liposomes (MPB-Lips), encapsulating the luminophore tris(2′,2-bipyridyl)ruthenium (II) complex ([Ru(bpy) 3 ] 2+ ), were developed as an electrochemiluminescence (ECL) signal amplifier and were applied to detect the model analyte streptavidin (SA) using the indirect competitive ECL method. The MPB-Lips were prepared by mixing various ratios of two different liposomes—one containing a phospholipid with a primary amine group and a biotinyl group (BIO/NH 2 -Lip) and one containing a phospholipid with an N -hydroxysuccinimide group (NHS-Lip) to allow binding between particles via amide bonds. Quartz crystal microbalance analysis using SA-modified gold-coated quartz crystals showed that the frequency shift values of MPB-Lips gradually decreased in the order BIO/NH 2 -Lip:NHS-Lip = 1:0
ISSN:	1618-2642 1618-2650 1618-2650
DOI:	10.1007/s00216-024-05532-1