Marine microalgae meal (Schizochytrium sp.) influence on intake, in vivo fermentation parameters and in vitro gas production and digestibility in sheep diets is dose-dependent

The objective of this study was to evaluate the dry matter intake (DMI), intake of nutrients and in vivo ruminal fermentation in wethers fed diets without or with 2 or 4 % of marine microalgae meal (Schizochytrium sp.) as a source of docosahexaenoic polyunsaturated fatty acid (DHA; C22:6 n-3), as well as the total gas production and digestibility of the experimental diets using in vitro techniques. Six Santa Inês wethers with a body weight of 55.6 ± 5.20 kg and 18 months of age fitted with a ruminal cannula, were used and housed in individual stalls. The experimental design consisted of a double 3 ×3 Latin square (three treatments and three periods) with each experimental period lasting 21 days (14 days for adaptation to diets, 5 days for sample collection and 2 days of blank time between periods), for a total of 63 days. Animals fed 4 % microalgae meal showed lower DMI (P