PagSWEET17a mediates sucrose allocation to xylem during wood formation in poplar

Wood is recognized as the primary storage tissue in trees. During the process of wood formation, the xylem actively assimilates carbon from photosynthetic tissues to satisfy its developmental requirements. The photosynthetic product, sucrose, provides precursors and energy for cell wall synthesis, a...

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Veröffentlicht in:Industrial crops and products 2024-12, Vol.222, p.119929, Article 119929
Hauptverfasser: Hao, Xinyi, Li, Jifu, Zhang, Li, Fan, Zhibin, Wang, Zheshu, Hou, Luxuan, Wang, Lijuan
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Sprache:eng
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Zusammenfassung:Wood is recognized as the primary storage tissue in trees. During the process of wood formation, the xylem actively assimilates carbon from photosynthetic tissues to satisfy its developmental requirements. The photosynthetic product, sucrose, provides precursors and energy for cell wall synthesis, and its transportation significantly impacts the yield and quality of wood. The radial transport is a critical step in the accumulation of photosynthetic products to xylem, yet research on the involved sugar transporters and their roles remains limited. In the present study, PagSWEET17a, a sugar transporter gene with pronounced expression in the xylem, was examined for its expression pattern, substrate specificity, subcellular localization, and the consequential effects on wood formation. The present findings revealed that PagSWEET17a exhibited specificity for sucrose transport, and has triple subcellular localizations, including the plasma membrane, tonoplast, and endoplasmic reticulum membrane. PagSWEET17a expressed in the phloem cells of the stem, as well as in the developing xylem vessels and fiber cells, suggesting its potential involvement in various steps of radial transport. By mediating the accumulation of carbon in the xylem, PagSWEET17a influenced the width of the xylem, as well as the thickness and composition of the secondary cell walls in xylem cells. These insights contribute to a deeper understanding of the molecular mechanisms underlying wood formation and highlight the importance of sugar transporters in this process. •PagSWEET17a is expressed in phloem cells and developing xylem cells.•PagSWEET17a mediates sucrose radial transport in xylem.•Mutation of PagSWEET17a leads to a reduction in the xylem area.•PagSWEET17a influences the thickness and composition of the secondary cell walls.
ISSN:0926-6690
DOI:10.1016/j.indcrop.2024.119929