Enhanced remediation of real agricultural runoff in surface-flow constructed wetlands by coupling composite substrate-packed bio-balls, submerged plants and functional bacteria: Performance and mechanisms

Import of agricultural runoff containing nutrients considerably contributes to eutrophication of receiving water bodies. Surface-flow constructed wetlands (SFCWs) are commonly applied for agricultural runoff purification, but the performance is usually unsatisfactory. In this study, suspended bio-balls filled with zeolite and iron-carbon (Fe-C) composite substrates, submerged macrophyte (Ceratophyllum demersum) and functional denitrifying bacteria were collectively added into SFCW microcosms to enhance the remediation efficiency for real agricultural runoff with high nutrient concentrations and low content of bioavailable organic matter. The bio-ball added SFCWs achieved notably higher pollutant removal efficiencies (21.1%, 80.2% and 47.5% for chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP), respectively) than the control (COD: 6.9%, TN: 64.4%, TP: 27.9%), because of the versatile functions of filling materials for pollutant removal. C. demersum plantation (COD: 44.2%, TN: 82.8% and TP: 53.7%) and functional bacteria inoculation (COD: 51.8%, TN: 85.8% and TP: 55.1%) further enhanced the efficiency of the SFCWs for agricultural runoff remediation. Bio-ball addition and C. demersum plantation significantly increased the humification degree and reduced the molecular weight of dissolved organic matter (DOM) in the agricultural runoff. Moreover, the two intensification measures also notably reduced organic and nitrogen contents in the wetland sediment. Remarkable distinction in bacterial community distribution patterns was observed in the SFCW sediment and filling substrates in bio-balls. Keystone genera including Clostridium_sensu_stricto_1 and Bacillus in the zeolite, Sphingomonas and Exiguobacterium in the Fe-C substrates and Sediminibacterium in the sediment might be critical for agricultural runoff remediation in the SFCW microcosms. The study highlights a high potential of the intensified SFCWs by these coupling measures for agricultural runoff remediation. [Display omitted] •Zeolite and Fe-C-filled bio-balls improved agricultural runoff purification in SFCWs.•Bioaugmentation by C. demersum and functional bacteria enhanced purification efficacy.•C. demersum presence increased humification and reduced molecular weight of DOM.•Microbial networks were complex in SFCW sediment and simple in Fe-C substrates.•Microbiota in fillers were more determined by deterministic processes.