Isolation and identification of NEAU-CP5: A seed-endophytic strain of B. velezensis that controls tomato bacterial wilt

Bacterial wilt of tomato caused by Ralstonia solanacearum is a critical soilborne disease that drastically reduces yield. In the current study, an endophytic strain NEAU-CP5 with strong antagonistic activity against R. solanacearum was isolated from tomato seeds and characterized. The strain was ide...

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Veröffentlicht in:Microbial pathogenesis 2024-07, Vol.192, p.106707, Article 106707
Hauptverfasser: Bing, Hui, Qi, Cuiping, Gu, Jinzhao, Zhao, Tianxin, Yu, Xiaoyan, Cai, Yang, Zhang, Yance, Li, Ailin, Wang, Xiangjing, Zhao, Junwei, Xiang, Wensheng
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Sprache:eng
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Zusammenfassung:Bacterial wilt of tomato caused by Ralstonia solanacearum is a critical soilborne disease that drastically reduces yield. In the current study, an endophytic strain NEAU-CP5 with strong antagonistic activity against R. solanacearum was isolated from tomato seeds and characterized. The strain was identified as Bacillus velezensis based on 16S rRNA gene and whole genome sequence analysis. NEAU-CP5 can secrete amylase, protease, and cellulase, and also produce known antibacterial metabolites, including cyclo (leucylprolyl), cyclo (phenylalanyl-prolyl), cyclo (Pro-Gly), 3-benzyl-2,5-piperazinedione, pentadecanoic acid, eicosane, 2-methyoic acid, isovaleric acid, dibuty phthalate, and esters of fatty acids (HFDU), which may be responsible for its strong antibacterial activity. Fourteen gene clusters associated with antibacterial properties were also identified in the whole genome sequence of NEAU-CP5. Pot experiment demonstrated that the application of 108 CFU/mL NEAU-CP5 on tomato plants significantly reduced the incidence of tomato bacterial wilt by 68.36 ± 1.67 %. NEAU-CP5 also increased the activity of defense-related enzymes (CAT, POD, PPO, SOD, and PAL) in tomato plants. This is the first report of an effective control of bacterial wilt on tomato plants by B. velezensis and highlights the potential of NEAU-CP5 as a potential biocontrol agent for the management of tomato bacterial wilt. •Bacillus velezensis NEAU-CP5 was an endophytic strain of tomato seeds.•NEAU-CP5 exhibited strong antagonistic activity against R. solanacearum.•NEAU-CP5 could produce amylase, protease, cellulase, and antibacterial metabolites.•NEAU-CP5 could significantly reduce the incidence of tomato bacterial wilt.•NEAU-CP5 could increase the activity of defense-related enzymes.
ISSN:0882-4010
1096-1208
1096-1208
DOI:10.1016/j.micpath.2024.106707