Study of nitrogen heterocycles as DNA/HSA binder, topoisomerase inhibitors and toxicological safety

Four new nitrogen-containing heterocyclic derivatives (acridine, quinoline, indole, pyridine) were synthesized and their biological properties were evaluated. The compounds showed affinity for DNA and HSA, with CAIC and CAAC displaying higher binding constants (Kb) of 9.54 × 10 and 1.06 × 10 , respe...

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Veröffentlicht in:International journal of biological macromolecules 2024-01, Vol.254 (Pt 3), p.127651-127651, Article 127651
Hauptverfasser: Dos Santos, Jéssica Celerino, Alves, Josival Emanuel Ferreira, de Azevedo, Rafael David Souto, de Lima, Maksuelly Libanio, de Oliveira Silva, Maria Regina, da Silva, Josefa Gerlane, da Silva, Jamire Muriel, de Carvalho Correia, Ana Carolina, do Carmo Alves de Lima, Maria, de Oliveira, Jamerson Ferreira, de Moura, Ricardo Olímpio, de Almeida, Sinara Mônica Vitalino
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Sprache:eng
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Zusammenfassung:Four new nitrogen-containing heterocyclic derivatives (acridine, quinoline, indole, pyridine) were synthesized and their biological properties were evaluated. The compounds showed affinity for DNA and HSA, with CAIC and CAAC displaying higher binding constants (Kb) of 9.54 × 10 and 1.06 × 10 , respectively. The fluorescence quenching assay (Ksv) revealed suppression values ranging from 0.34 to 0.64 × 10  M for ethidium bromide (EB) and 0.1 to 0.34 × 10  M for acridine orange (AO). Molecular docking confirmed the competition of the derivatives with intercalation probes at the same binding site. At 10 μM concentrations, the derivatives inhibited topoisomerase IIα activity. In the antiproliferative assays, the compounds demonstrated activity against MCF-7 and T47-D tumor cells and nonhemolytic profile. Regarding toxicity, no acute effects were observed in the embryos. However, some compounds caused enzymatic and cardiac changes, particularly the CAIC, which increased SOD activity and altered heart rate compared to the control. These findings suggest potential antitumor action of the derivatives and indicate that substituting the acridine core with different cores does not interfere with their interaction and topoisomerase inhibition. Further investigations are required to assess possible toxicological effects, including reactive oxygen species generation.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.127651